DNA ISOLATION - Isolation of Plasmid DNA / Isolation of Bacterial Genomic DNA / Isolation of Yeast Genomic DNA / Isolation of Fungal Genomic DNA / Isolation of Genomic DNA from Blood / Isolation of DNA from Animal Cells / Isolation of Genomic DNA from Eukaryotic Tissues / Isolation of Plant DNA using CTAB Extraction Method / Isolation of Chloroplast DNA / Mitochondrial DNA Isolation / Phenol Chloroform Extraction of DNA / Ethanol Precipitation of DNA / RNA ISOLATION - Isolation of Total RNA from Bacterial Cells / Isolation of Total RNA from Plant Tissues / Hot Phenol Isolation of RNA from Plant Tissues / Acid Phenol Extraction of RNA / Messenger RNA Isolation or Poly (A) RNA Isolation / WORKING WITH DNA - Storage / Purification / Concentration / Spectrophotometric Determination of Nucleic Acid Purity and Concentration / Fluorescent Quantification of DNA / Quantification of DNA using Diphenylamine (DPA) Assay / MOLECULAR BIOLOGY METHODS - Restriction Enzyme Digestion of DNA / DNA Ligation / Agarose Gel Electrophoresis of DNA / Elution of DNA Fragments from Agarose / Phenol Purification of DNA from Low Melting Agarose / Southern Blotting / Agarose Gel Electrophoresis of RNA / Northern Blotting / Cloning / Polymerase Chain Reaction (PCR)(In Vitro Amplification of DNA) / SDS–Polyacrylamide Gel Electrophoresis / Western Blotting (Immunoblotting) / Iso-electric Focusing (IEF) of Proteins / 2D Gel Electrophoresis (2D PAGE) / Trypsin Digestion of Protein Gel / Protein Dialysis / Enzyme (Esterase) Gel Electrophoresis / SOME USEFUL INFORMATION FOR METHODS IN MOLECULAR BIOLOGY - Antibiotic Concentration in Media / E. coli Growth Curve / Storage of Bacterial Strains in Stab Agar / Storage of Bacterial Strains in Glycerol Solution / Decontamination of Ethidium Bromide (EtBr) Solutions / Preparation of Solutions / Glassware and Plasticware / Disposal of Buffers and Chemicals / Autoclave Operating Procedures / Safety Procedures / PREPARATION OF SOLUTIONS / References
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